Measurement

Part:BBa_K1379003:Design

Designed by: Ng Siu Wang Edward, Jason Jang, Joo Min Jung   Group: iGEM14_Hong_Kong_HKUST   (2014-09-22)

PcomFA-E0240 (Measurement Kit for PcomFA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 831

Design Notes

None

Source

- PcomFA primers sequence was obtained from Wellcome Trust Sanger Institute, a British genomics and genetics research institute.
- iGEM 2014 Hong_Kong_HKUST Team has cloned PcomFA from S. pneumoniae strain NCTC7465 by PCR, followed by restriction-ligation with BBa_E0240 in pSB1C3 backbone.

References